In Vitro Interaction of Heavy Metals with Ouabain Receptors in Rat Brain Microsomes
- 1 January 1993
- journal article
- research article
- Published by Taylor & Francis in Drug and Chemical Toxicology
- Vol. 16 (1) , 101-110
- https://doi.org/10.3109/01480549309038663
Abstract
This study investigates the influence of heavy metals on ouabain-binding in presence of thiol (sulfhydryl) compounds. The data on in vitro effects of mercury (Hg), lead (Pb) and cadmium (Cd) showed significant inhibition of 3H-ouabain binding to microsomal membrane in a concentration-dependent manner. Maximum inhibition of 3H-ouabain binding was observed at 1 microM for Hg and 100 microM each for Pb and Cd. Preincubation with monothiol (L-cysteine or glutathione) or dithiol (dithiothreitol) protected inhibition of 3H-ouabain binding to the membranes by Hg or Pb. Dithiol but not monothiols partially protected Cd-inhibition. The present data confirm that the heavy metals interact with ouabain receptors in a manner similar to SH-blocking agents and protection of metal-inhibited 3H-ouabain binding by thiol compounds is metal specific.Keywords
This publication has 13 references indexed in Scilit:
- Effect of mercuric chloride on the kinetics of cationic and substrate activation of the rat brain microsomal ATPase systemBiochemical Pharmacology, 1990
- Symposium on Neurotoxicology of Heavy Metals IIFundamental and Applied Toxicology, 1987
- Effects of tricyclohexylhydroxytin on the kinetics of adenosine triphosphatase system and protection by thiol reagentsJournal of Biochemical Toxicology, 1987
- Effect of chlordecone on pH and temperature dependent substrate activation kinetics of rat brain synaptosomal ATPasesBiochemical Pharmacology, 1983
- Effects of Cd2+, Mn2+, and Al3+ on rat brain synaptosomal uptake of noradrenaline and serotoninJournal of Inorganic Biochemistry, 1982
- Effect of transitional metal ions on (Na+ + K+) ATPase activity and the uptake of norepinephrine and choline by brain synaptosomesLife Sciences, 1973
- Biochemical Effects of Mercury, Cadmium, and LeadAnnual Review of Biochemistry, 1972
- The nature of the transport ATPase-digitalis complex. I. Formation and reversibility in the presence and absence of a phosphorylated enzymeBiochemical and Biophysical Research Communications, 1971
- FRACTIONATION OF OLFACTORY TISSUE HOMOGENATES. ISOLATION OF A CONCENTRATED PLASMA MEMBRANE FRACTIONJournal of Neurochemistry, 1969
- Tritiated Digoxin Binding to (Na + + K + )-Activated Adenosine Triphosphatase: Possible Allosteric SiteScience, 1968