INHIBITION OF AUTOLOGOUS MIXED LYMPHOCYTE-REACTION BY MONOCLONAL-ANTIBODIES SPECIFIC FOR THE BETA-CHAIN OF HLA-DR ANTIGENS
- 1 January 1984
- journal article
- research article
- Vol. 53 (1) , 79-86
Abstract
Recent studies using rabbit antisera to the separated HLA-DR .alpha. and .beta. subunits have suggested that .alpha. chain-specific, but not .beta. chain-specific, antisera inhibit T cell proliferative responses in primary and secondary human autologous mixed lymphocyte reaction (AMLR). In the present study, with the aid of sequential co-precipitation assays and Western blotting methods, a monoclonal rat alloantibody 1E4, specific for the .beta. chain of rat class II molecules carrying an Ia determinant Ba-2.7, was characterized to recognize a monomorphic determinant located on the .beta. chain of DR antigens. This antibody and a murine monoclonal antibody HU-4, also specific for the .beta. chain of DR antigens, strongly inhibited both primary and secondary AMLR through a mechanism distinct from an antibody-dependent cell-mediated cytotoxicity reaction. Evidently, the inhibition of AMLR is not a unique feature of DR .alpha.-specific antibodies.This publication has 22 references indexed in Scilit:
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