THE FLUORIMETRIC MICRODETERMINATION OF GLYOXYLIC ACID IN BLOOD, URINE AND BACTERIAL EXTRACTS

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Abstract
A spectrophotofluorimetric method for the determination of glyoxylic acid in biological materials is described. The method is based on the reaction between glyoxylic acid and resorcinol in acid solution, a fluorescent complex being obtained on the subsequent addition of alkali. The reaction was found to be sensitive and highly specific, the minimum detectable amount of glyoxylic acid being 1-35 x 10-8 mole. The urinary excretion of glyoxylic acid by ten normal adults ranged from 1.4 to 4.7 mg/24 hr. Small but measurable amounts of glyoxylic acid were found in cell-free extracts of Pseudomonas oxalaticus OX1 grown on oxalic acid as a source of carbon. No glyoxylic acid was detected in human serum.