Preparation of uricase

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Abstract
Essential features of a new procedure are: the prepn. of an extract from ox-kidney mince incubated for 16-18 hr. with borate buffer, pH 10, in the presence of n-butanol; purification by Ca phosphate gel at alkaline and acid pH; repeated precipitations of the enzyme with half-saturated ammonium sulphate; and separation from inactive proteins by dialysis. From 1 kg. ox kidneys it is possible to obtain 620 mg. of a prepn., with a QO2 value not less than 700. Uricase prepared according to the present method was free from catalase at an early stage of purification. The suitability of this enzyme prepn. for the purpose of uric acid detn. is discussed.