Chemical Synthesis of Restriction Enzyme Recognition Sites Useful for Cloning
- 8 April 1977
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 196 (4286) , 177-180
- https://doi.org/10.1126/science.847463
Abstract
By a triester chemical synthesis method, three decameric DNA's have been made; these act as substrates for several restriction endonucleases, including Eco RI, Bam I, and Hind III. These homogenous decamers form duplexes that can be efficiently blunt-end ligated to themselves or to other DNA molecules by the action of T4 DNA ligase and thus are useful tools for molecular cloning experiments.This publication has 10 references indexed in Scilit:
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