Clones of Individual Repetitive Sequences from Sea Urchin DNA Constructed with Synthetic Eco RI Sites
- 8 April 1977
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 196 (4286) , 197-200
- https://doi.org/10.1126/science.847467
Abstract
Interspersed repetitive sequences were isolated from sea urchin DNA by renaturing to low Cot followed by treatment with nuclease SI. Synthetic Eco RI sites were ligated onto the repetitive sequence elements, which were then inserted at the Eco RI site of plasmid RSF2124 and cloned. The repetitive sequences can be excised from the plasmid with Eco RI for further study.This publication has 11 references indexed in Scilit:
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