?Cytogenetic? studies of spermatids of mice carrying Cattanach's translocation by flow cytometry
- 1 January 1979
- journal article
- research article
- Published by Springer Nature in Chromosoma
- Vol. 74 (2) , 141-151
- https://doi.org/10.1007/bf00292269
Abstract
The DNA content of spermatids of mice carrying Cattanach's translocation has been measured with high precision by flow cytometry. The observation that the two peaks of DNA content in the haploid region of the DNA histograms represent X-and Y-bearing spermatids was tested and confirmed. Using flow cytometry, the difference in DNA content between the X and Y chromosomes in these mice was measured to be 5.2±0.1% of the total haploid genome as compared to 3.4±0.1% in normal mice. These results demonstrated the precision of flow cytometry for cytogenetic studies. Additional information on spermatogenesis in mice bearing Cattanach's translocation was obtained and showed a gradual loss of cells during spermatogenesis in those bearing the balanced form of the translocation.Keywords
This publication has 16 references indexed in Scilit:
- Effects of Acute Gamma-Irradiation on Spermatogenesis as Revealed by Flow CytometryActa Radiologica: Oncology, 1980
- Cell-cycle phase-dependence of drug-induced cycle progression delay.Journal of Histochemistry & Cytochemistry, 1979
- Resolution of X and Y spermatids by pulse cytophotometryNature, 1978
- Increased variability in nuclear DNA content of testis cells and spermatozoa from mice with irregular meiotic segregationMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1978
- Flow cytometry of DNA in mouse sperm and testis nucleiMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1978
- Measurement of mammalian sperm deoxyribonucleic acid by flow cytometry. Problems and approaches.Journal of Histochemistry & Cytochemistry, 1977
- DNA-fluorometry of mammalian spermHistochemistry and Cell Biology, 1977
- Flow-cytogenetics: sources of DNA content variation among euploid individualsCytogenetic and Genome Research, 1977
- Centrifugal elutriation: Separation of spermatogenic cells on the basis of sedimentation velocityJournal of Cellular Physiology, 1975
- Cattanach's Translocation: Cytological Characterization by Quinacrine Mustard StainingProceedings of the National Academy of Sciences, 1971