Studies on plant cell fusion by dextran: Effects of pH, inorganic salts and electrical stimulus.

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Abstract
To improve the dextran method for plant cell fusion, the effects of pH, inorganic salts and electrical stimulus on fusion were investigated using protoplasts [Brassica pekinensis, Petunia hybrida, Nicotiana tabacum, Daucus carota] aggregated by dextran solutions. Fusion frequency was enhanced by high pH conditions or by electrical stimulus. Fusion took place during dilution of dextran-protoplast mixtures with Mn2+ and Ca2+ solutions. Protoplasts treated by the dextran method developed into colonies after 3-4 wk. The dextran method is useful for understanding the fusion methanism and for inducing somatic hybrids practically.