Preparation and properties of monomercurated tRNA
- 1 October 1983
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 136 (1) , 147-150
- https://doi.org/10.1111/j.1432-1033.1983.tb07718.x
Abstract
Monomercurated tRNA was prepared by exhaustive treatment of mercaptoethanol on polymercurated tRNA under non-denaturing conditions. The remaining Hg atom is stable toward UV (254 nm) irradiation up to absorption of 100 quanta/nucleotide, but could be easily removed by mercaptoethanol under denaturing conditions. Monomercurated tRNA quantitatively binds to thiopropyl-Sepharose and can be completely desorbed by mercaptoethanol-containing solutions. Monomercurated tRNA is similar to the starting tRNA preparation in efficiency and specificity of aminoacylation and in factor-dependent polypeptide synthesis in an Escherichia coli in vitro system.This publication has 14 references indexed in Scilit:
- Sulfhydrylcellulose: a new medium for chromatography of mercurated polynucleotidesBiochemistry, 1981
- Ribosomal proteins contacting with deacylated tRNA in the S‐site of the translating ribosomeFEBS Letters, 1981
- Study of the interaction between yeast tRNAPhe and yeast phenylalanyl-tRNA synthetase by monochromatic ultraviolet irradiation at various wavelengths Advantages and limits of the methodBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1981
- Fractionation of Escherichia coli isoaccepting tRNA species by Sepharose 4B Column chromatographyAnalytical Biochemistry, 1979
- The Mechanism of Codon-Anticodon Interaction in Ribosomes. Quantitative Study of Codon-Dependent Binding of tRNA to the 30-S Ribosomal Subunits of Escherichia coliEuropean Journal of Biochemistry, 1978
- Factor-free (“Non-enzymic”) and factor-dependent systems of translation of polyuridylic acid by Escherichia coli ribosomesJournal of Molecular Biology, 1976
- Direct covalent mercuration of nucleotides and polynucleotidesBiochemistry, 1975
- Mercurated polynucleotides. New probes for hybridization and selective polymer fractionationBiochemistry, 1975
- The Synthesis and Enzymatic Polymerization of Nucleotides Containing Mercury: Potential Tools for Nucleic Acid Sequencing and Structural AnalysisProceedings of the National Academy of Sciences, 1973
- The isolation and fractionation of soluble ribonucleic acidJournal of Molecular Biology, 1962