Cryoprecipitates and Immune Complexes: Differentiation between Cold Insoluble Immune Complexes and Nonspecific Cryoprecipitates
- 1 January 1976
- journal article
- research article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 52 (1-4) , 169-174
- https://doi.org/10.1159/000231678
Abstract
Cold insoluble circulating immune complexes occurred in 77% of rabbits immunized with 125I BSA. 9.8% of the total cryoprotein was 125I BSA. This represented 43% of the serum antigen. Tripling the antigen concentration in vitro decreased the amount of cold insoluble antigen by as much as 83%. In nonspecific cryoprecipitates only 0.4% of the total cryoprotein was 125I BSA. This represented only 0.028% of the serum antigen. Tripling of the antigen concentration in vitro increased the amount of cold insoluble antigen. Cold insoluble circulating immune complexes occur at a critical antigen-antibody ratio and can be differentiated from nonspecific cryoprecipitates.Keywords
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