STUDIES ON DETERMINATIONS OF ALPHA-AMYLASE WITH PARA-NITROPHENYL-ALPHA-D-MALTOTETRAOSIDE

  • 1 January 1982
    • journal article
    • research article
    • Vol. 20  (11) , 799-805
Abstract
Nitrophenylmaltodextrins are [human] .alpha.-amylase substrates which allow continuous determination with a zero order kinetics over a period of at least 10 min, without deviations from linearity. Only 1 auxillary enzyme is necessary. Practicability and clinical evidence of .alpha.-amylase determinations by means of p-nitrophenyl-.alpha.-D-maltotetraoside are demonstrated. The interserial precison of 0.84% cannot conceal an only moderate correlation with previous methods. This fact, however, does not negate the advantages.