• 1 January 1980
    • journal article
    • research article
    • Vol. 26  (7) , 846-853
Abstract
Four kinetic amylase procedures are evaluated with respect to kinetics, analytical range, blank rates, reagent stability, reagent impurities, interfering substances and intrinsic sensitivities. Each of the methods has its own unique advantages and disadvantages. A preliminary discussion of some alternative methods in which glycosidic p-nitrophenyl .alpha.-oligosaccharides are substrates is included. [The determination of .alpha.-amylase (1.4-.alpha.-D-glucan glucanohydrolase; EC 3.2.1.1) activity in [human] serum is a widely accepted test for the diagnosis of acute pancreatitis, and for obstruction of the pancreatic duct.].