Changes in the Surface of Virus-Infected Cells Recognized by Cytotoxic T Cells.

Publisher Website
Google Scholar
Abstract
Infection of [mouse] cells with ectromelia or lymphocytic choriomeningitis (LCM) virus in the presence of 2-deoxy-D-glucose (2-DOG) inhibited by up to 70% the extent to which the infected cells become susceptible to virus-specific cell-mediated lysis. The concentration of 2-DOG used had little effect on the extent of total protein synthesis (incorporation of [35S]methionine) but inhibited (up to 25%) glycoprotein synthesis, as measured by incorporation of [3H]fucose. This suggested that glycoprotein synthesis was a necessary event for infected cells to become susceptible to T[thymus-derived]-cell-mediated lysis. The profiles (polyacrylamide gel electrophoresis) of newly synthesized, cellular glycoproteins from uninfected and ectromelia-infected cells in the presence and absence of 2-DOG were compared and were very complex, with only minor changes. When convalescent serum from infected mice was used to isolate newly synthesized components from the cell surface shortly after infection, it showed 4 main species ranging in size from 25,000-70,000 daltons. Also 2-DOG inhibited production of these by 70%, thus corresponding to the biological data.