A method for the isolation of phage mutants altered in their response to lysogenic induction
- 1 January 1976
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 145 (2) , 165-167
- https://doi.org/10.1007/bf00269589
Abstract
Phage λcl + gives clear plaques whereas phage λcIind - gives turbid plaques on a lawn of a mutant strain of E. coli K12. This strain, called STS, carries mutation spr in a tif sfi genetic background. I hypothesize that upon temperate phage infection, STS bacteria spontaneously inactivate phage repressor by the same mechanism involved in normal lysogenic induction which results in obligatory lytic growth of λ+. The use of the STS mutant facilitates the isolation and genetic analysis of phage mutants with an abnormal response to lysogenic induction.This publication has 20 references indexed in Scilit:
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