Comparison of methods used in commercial kits for the assay of serum vitamin B12
- 23 September 1983
- journal article
- research article
- Published by Wiley in Clinical and Laboratory Haematology
- Vol. 5 (3) , 271-277
- https://doi.org/10.1111/j.1365-2257.1983.tb01364.x
Abstract
Fourteen commercial kits for serum vitamin B12 assay have been assessed. The analytical performance and clinical correlation were used to rank the methods employed for the extraction of the vitamin from its binders and for the separation of 'bound' from 'unbound' counts. The introduction of extraction without boiling and the separation by attachment of the vitamin B12 binder to a solid matrix have not been shown to be generally better or worse than conventional methods.Keywords
This publication has 7 references indexed in Scilit:
- High serum vitamin B12 binding capacity as a marker of the fibrolamellar variant of hepatocellular carcinoma.BMJ, 1983
- Assessment of a radioisotopic assay for vitamin B12using an intrinsic factor preparation with R proteins blocked by vitamin B12analoguesJournal of Clinical Pathology, 1982
- Comparative studies of a new commercial kit for the estimation of vitamin B12 in serum.Journal of Clinical Pathology, 1981
- Erroneously Low Results Due to High Nonspecific Binding Encountered with a Radioassay Kit That Measures “True” Serum Vitamin B12American Journal of Clinical Pathology, 1980
- An evaluation of commercial radioisotope methods for the determination of folate and vitamin B12.Journal of Clinical Pathology, 1980
- Use of Dextran-Coated Charcoal to Separate Antibody-Bound from Free Hormone: A CritiqueJournal of Clinical Endocrinology & Metabolism, 1973
- Recognition and Resolution of Errors in the Radioisotopic Assay of Serum Vitamin B12American Journal of Clinical Pathology, 1970