Growth inhibitory effect of recombinant ? and ? interferon on human glioma cells

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Abstract
Growth inhibitory activity of recombinant α and β interferon on two human glioma cell lines, EFC-2 and KE cells, was determined by two different growth assays. Recombinant β interferon showed β slight growth inhibitory effect on EFC-2 cells at day 3, and maximum inhibition was seen on day 6 with an ID50 of 50 U/ml. Recombinant α interferon showed no significant growth inhibition at any concentration. KE cells were resistant to both recombinant α and β interferon. The growth inhibitory activity of recombinant β interferon on EFC-2 cells was not blocked by recombinant α interferon, although recombinant α and β interferons shared same receptors on EFC-2 cells. Addition of DFMO (α-difluoromethylornithine) to interferon in the media showed additive effect rather than synergistic effect in growth inhibition of glioma cells. Out of 7 glioma cell lines tested, 4 showed heterogeneous sensitivity to recombinant β interferon, and all were resistant to recombinant α interferon. These results suggest β differential sensitivity of EFC-2 cells to recombinant β interferon, as well as a heterogeneous sensitivity to recombinant β interferon among different glioma cell lines.