The preparation of human spermatozoa for analytical and scanning electron microscopy

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Abstract
SUMMARY: Human spermatozoa have been prepared for electron microscopy comparing methods of specimen preparation and support mechanisms which enable investigations of elemental composition related to changes in surface morphology. A technique combining SEM of critical‐point dried, gold coated spermatozoa mounted on glass cover slips with analytical STEM of air dried, carbon coated spermatozoa mounted on formvar coated nickel grids proved most reliable and reproducible. The relative advantages and disadvantages of the methods, and the results obtained from them, are discussed.