Actin and calmodulin coexist in the equatorial segment of ejaculated boar sperm
- 31 May 1987
- journal article
- research article
- Published by Wiley in Gamete Research
- Vol. 17 (2) , 97-105
- https://doi.org/10.1002/mrd.1120170202
Abstract
The direct overlay technique with iodinated calmodulin on boar sperm fractions evidenced a strong bond in the 45,000 molecular weight, which is the region recognized by anti‐actin antibodies. This result and the close codistribution of the two staining patterns for calmodulin and actin with immunofluorescence and immunoelectronmicroscopy suggest a possible interaction between calmodulin and actin in boar sperm.Keywords
This publication has 28 references indexed in Scilit:
- Immunoelectron microscopic localization of calmodulin and phospholipase A2 in spermatozoa. I.Journal of Histochemistry & Cytochemistry, 1986
- Immunoelectronmicroscopic localization of actin in ionophore-treated boar spermCell Biology International Reports, 1986
- Ca+ + localization in boar spermatozoa by the pyroantimonate technique and X‐ray microanalysisJournal of Experimental Zoology, 1986
- Identification of calmodulin-binding proteins in chicken embryo fibroblasts.The Journal of cell biology, 1984
- Plasma-membrane diversity in a highly polarized cell.The Journal of cell biology, 1982
- Actin in Human SpermatozoaBiology of Reproduction, 1982
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981
- Immunocytochemical and biochemical evidence for the presence of calmodulin in bull sperm flagellum isolation and characterization of sperm calmodulinBiochimica et Biophysica Acta (BBA) - General Subjects, 1981
- Actin in mammalian sperm headsJournal of Experimental Zoology, 1978
- High levels of a calcium-dependent modulator protein in spermatozoa and its similarity to brain modulator proteinBiochemical and Biophysical Research Communications, 1978