Transferable tetracycline resistance in Clostridium perfringens strains of porcine origin

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Abstract
These studies represent the first systematic survey of the incidence of conjugative antibiotic resistance in Clostridium perfringens. Ninety-two antibiotic-resistant porcine strains were examined to see if they could donate their antibiotic-resistance determinants to sensitive recipient strains. Fifteen of the 89 tetracycline-resistant strains transferred their tetracycline resistance in mixed-plate mating experiments but no transfer of macrolide–lincosamide resistance was detected. The efficiencies of transfer of tetracycline resistance varied from 1.3 × 10−3 to 1.9 × 10−6 transconjugants per donor cell. Significantly higher transfer efficiencies were observed when both the donor and recipient strains were derivatives of strain CW 362. These values ranged from 3.7 × 10−1 to 4.6 × 10−2 transconjugants per donor cell. This high frequency transfer system should prove invaluable for further genetic studies on this microorganism.