RNA polymerase II transcription termination is mediated specifically by protein binding to a CCAAT box sequence.
Open Access
- 1 November 1989
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 9 (11) , 5254-5259
- https://doi.org/10.1128/mcb.9.11.5254
Abstract
A region in the adenovirus major late promoter (MLP) containing a CCAAT consensus sequence can direct transcription termination of RNA polymerase II, a mechanism that possibly prevents transcriptional interference from upstream genes. Using a chimeric plasmid template that contains the MLP directing expression of the simian virus 40 early region, we showed that an inserted oligonucleotide containing only 13 base pairs of MLP sequences, including the CCAAT box, is capable of inducing transcription termination in an orientation-dependent, position-independent manner. Point mutations within the CCAAT-specific protein-binding site abolished this effect, while a base substitution outside of this region did not affect termination. These data suggest that termination is mediated by a CCAAT box-binding protein. Several other transcription factor-binding sites do not, however, cause termination, suggesting that this may be a relatively specific property of a CCAAT-binding protein.This publication has 37 references indexed in Scilit:
- How RNA polymerase II terminates transcription in higher eukaryotesTrends in Biochemical Sciences, 1989
- Selective Activation of Transcription by a Novel CCAAT Binding FactorScience, 1988
- A functional mRNA polyadenylation signal is required for transcription termination by RNA polymerase II.Genes & Development, 1988
- Trans-acting protein factors and the regulation of eukaryotic transcription: lessons from studies on DNA tumor viruses.Genes & Development, 1988
- A multiplicity of CCAAT box-binding proteinsCell, 1987
- TRANSCRIPTION TERMINATION AND THE REGULATION OF GENE EXPRESSIONAnnual Review of Biochemistry, 1986
- A repeated 18 bp sequence motif in the mouse rDNA spacer mediates binding of a nuclear factor and transcription terminationCell, 1986
- Structure and function of the S1 nuclease-sensitive site in the adenovirus late promoterCell, 1986
- Transcription of mouse rDNA terminates downstream of the 3′ end of 28S RNA and involves interaction of factors with repeated sequences in the 3′ spacerCell, 1985
- Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5Journal of General Virology, 1977