Nuclear and Nucleolar Size Changes and Nuclear Pore Frequency in Cultured Explants of Jerusalem Artichoke Tubers (Helianthus tuberosus L.)

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Abstract
Nucleolar and nuclear envelope size changes in cultured explants of H. tuberosus L. were studied prior to the first mitotic division. Using the technique of nuclear isolation to facilitate measurements results were obtained showing an almost immediate increase in nuclear envelope surface area, while nucleolar volume showed no appreciable increase until 4 h after excision. The sharp increase in nucleolar volume shown at this time reaches a maximum at 18 h which is maintained until mitosis occurs. The frequency of nuclear pores remains constant. These results are discussed in the light of previous work on levels of RNA throughout the activation process.

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