Recombinational DNA double-strand breaks in mice precede synapsis
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- 1 March 2001
- journal article
- Published by Springer Nature in Nature Genetics
- Vol. 27 (3) , 271-276
- https://doi.org/10.1038/85830
Abstract
In Saccharomyces cerevisiae, meiotic recombination is initiated by Spo11-dependent double-strand breaks (DSBs), a process that precedes homologous synapsis. Here we use an antibody specific for a phosphorylated histone (gamma-H2AX, which marks the sites of DSBs) to investigate the timing, distribution and Spo11-dependence of meiotic DSBs in the mouse. We show that, as in yeast, recombination in the mouse is initiated by Spo11-dependent DSBs that form during leptotene. Loss of gamma-H2AX staining (which in irradiated somatic cells is temporally linked with DSB repair) is temporally and spatially correlated with synapsis, even when this synapsis is 'non-homologous'.Keywords
This publication has 37 references indexed in Scilit:
- Meiotic Synapsis in the Absence of RecombinationScience, 1998
- Meiosis-Specific DNA Double-Strand Breaks Are Catalyzed by Spo11, a Member of a Widely Conserved Protein FamilyCell, 1997
- Meiosis in Carriers of Heteromorphic Bivalents: Sex Differences and Implications for Male FertilityChromosome Research, 1997
- Change of Karyoskeleton during Mammalian Spermatogenesis: Expression Pattern of Nuclear Lamin C2 and Its RegulationExperimental Cell Research, 1996
- Presynaptic association of Rad51 protein with selected sites in meiotic chromatin.Proceedings of the National Academy of Sciences, 1996
- Male mice defective in the DNA mismatch repair gene PMS2 exhibit abnormal chromosome synapsis in meiosisCell, 1995
- Sex chromosomes, recombination, and chromatin conformationChromosoma, 1993
- Univalent sex chromosomes in spermatocytes of Sxr-carrying miceChromosoma, 1988
- Identification of a family of human centromere proteins using autoimmune sera from patients with sclerodermaChromosoma, 1985
- Synaptonemal complex analysis of mouse chromosomal rearrangementsChromosoma, 1981