Radiation inactivation analysis of kidney microvillar peptidases

Publisher Website
Google Scholar
Abstract
Five membrane peptidases were studied by radiation inactivation analysis of pig kidney microvillar membranes. One heterodimeric enzyme, γ-glutamyl transferase, presented a target size corresponding to the dimeric M r. The other enzymes are known to be homodimers. Three of these, aminopeptidase A, aminopep-tidase N and dipeptidyl peptidase IV, gave results clearly indicating the monomer to be the target and, hence, in this group the association of the subunits was not essential for activity. The target size for endopep-tidase-24.11 was intermediate between those for monomer and dimer and its functional state was not resolved by the experiments.