Monitoring the Growth of a Bacteria Culture by MALDI-MS of Whole Cells
- 9 April 1999
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 71 (10) , 1990-1996
- https://doi.org/10.1021/ac981196c
Abstract
We have probed the time evolution of a growing bacteria culture by extracting samples periodically and performing matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) on whole cells. The mass spectra generated by this method contain tens of peaks in the 3−11-kDa mass range. Cultures of E. coli strain K-12 were grown in two types of containers and at two nutrient concentrations and sampled periodically from 6 to 84 h after inoculation. The relative intensities of several of the stronger peaks vary quite dramatically as a function of time. These temporal characteristics must be taken into account when MALDI-MS is applied to identify bacteria. The results also suggest that MALDI-MS can be used to follow the aging of a bacteria culture.Keywords
This publication has 14 references indexed in Scilit:
- Fingerprint matching ofE.coli strains with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of whole cells using a modified correlation approachRapid Communications in Mass Spectrometry, 1998
- The rapid identification of intact microorganisms using mass spectrometryNature Biotechnology, 1996
- Determination of Bacterial Protein Profiles by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry with High-performance Liquid ChromatographyRapid Communications in Mass Spectrometry, 1996
- Rapid Identification of Intact Whole Bacteria Based on Spectral Patterns using Matrix-assisted Laser Desorption/Ionization with Time-of-flight Mass SpectrometryRapid Communications in Mass Spectrometry, 1996
- High resolution matrix‐assisted laser desorption/ionization time‐of‐flight analysis of single‐stranded DNA of 27 to 68 nucleotides in lengthRapid Communications in Mass Spectrometry, 1995
- Separation and identification of organic gunshot and explosive constituents by micellar electrokinetic capillary electrophoresisAnalytical Chemistry, 1991
- Direct analysis of bacterial fatty acids by Curie-point pyrolysis tandem mass spectrometryAnalytical Chemistry, 1990
- Constant neutral loss scanning for the characterization of bacterial phospholipids desorbed by fast atom bombardmentAnalytical Chemistry, 1988
- Profiling of bacteria by fast atom bombardment mass spectrometryAnalytical Chemistry, 1987
- Identification of bacteria using mass spectrometryAnalytical Chemistry, 1975