Enzymatic amplification of electroimmunoprecipitates in agarose gels with horseradish peroxidase‐labeled protein A

Publisher Website
Google Scholar
Abstract
Electroimmunoprecipitates in agarose gels were amplified with protein A‐horseradish peroxidase (HRP) conjugate. The method is simple and detected quantitatively as low as 0.5 ng of human α‐fetoprotein (AFP) in electroimmunodiffusion. The alternative methods employing the second antibody, labeled initially or afterwards with HRP, failed to stain AFP immunoprecipitates. Direct application of anti‐AFP immunoglobulins‐HRP in electroimmunodiffusion produced less contrasted colored precipitates. Immunoprecipitates of other proteins were similarly visualized by the protein A‐HRP method, avoiding the use of specifically labeled antibody.

This publication has 16 references indexed in Scilit: