Replication of Varicella-Zoster Virus in Human Skin Organ Culture
Open Access
- 1 September 2005
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 79 (17) , 11501-11506
- https://doi.org/10.1128/jvi.79.17.11501-11506.2005
Abstract
Varicella-zoster virus (VZV) infection is restricted to humans, which hinders studies of its pathogenesis in rodent models of disease. To facilitate the study of VZV skin tropism, we developed an ex vivo system using human fetal skin organ culture (SOC). VZV replication was analyzed by plaque assay, transmission electron microscopy, and histology. The yield of infectious VZV from SOC increased ∼100-fold over 6 days, virions were abundant, and lesions developed that contained VZV antigens and resembled varicella and zoster lesions. The SOC system for VZV replication has applications for testing virus mutants and antiviral drugs.Keywords
This publication has 37 references indexed in Scilit:
- Roscovitine, a Cyclin-Dependent Kinase Inhibitor, Prevents Replication of Varicella-Zoster VirusJournal of Virology, 2004
- The Immediate-Early 63 Protein of Varicella-Zoster Virus: Analysis of Functional Domains Required for Replication In Vitro and for T-Cell and Skin Tropism in the SCIDhu Model In VivoJournal of Virology, 2004
- Mutational Analysis of Open Reading Frames 62 and 71, Encoding the Varicella-Zoster Virus Immediate-Early Transactivating Protein, IE62, and Effects on Replication In Vitro and in Skin Xenografts in the SCID-hu Mouse In VivoJournal of Virology, 2003
- Construction of Varicella-Zoster Virus Recombinants from Parent Oka Cosmids and Demonstration that ORF65 Protein Is Dispensable for Infection of Human Skin and T Cells in the SCID-hu Mouse ModelJournal of Virology, 2003
- Differentiation of Varicella-Zoster Virus ORF47 Protein Kinase and IE62 Protein Binding Domains and Their Contributions to Replication in Human Skin Xenografts in the SCID-hu MouseJournal of Virology, 2003
- Microarray Analysis of Host Cell Gene Transcription in Response to Varicella-Zoster Virus Infection of Human T Cells and Fibroblasts In Vitro and SCIDhu Skin Xenografts In VivoJournal of Virology, 2003
- Promoter Sequences of Varicella-Zoster Virus Glycoprotein I Targeted by Cellular Transactivating Factors Sp1 and USF Determine Virulence in Skin and T Cells in SCIDhu Mice In VivoJournal of Virology, 2003
- Tropism of Varicella-Zoster Virus for Human Tonsillar CD4+T Lymphocytes That Express Activation, Memory, and Skin Homing MarkersJournal of Virology, 2002
- Glycoprotein I of Varicella-Zoster Virus Is Required for Viral Replication in Skin and T CellsJournal of Virology, 2002
- HISTOPATHOLOGY OF THE DISEASES OF THE SKINThe Lancet Healthy Longevity, 1896