GENETICS OF PLT RESPONSE .2. HLA-DRW IS A MAJOR PLT-STIMULATING DETERMINANT

  • 1 January 1979
    • journal article
    • research article
    • Vol. 123  (1) , 37-42
Abstract
PLT [primed lymphocyte typing] response is restricted by the HLA-D region. The role of HLA-DRw in PLT restimulation was studied. Haplotype-primed intrafamily PLT cells were made against specificities HLA-DRw1, HLA-DRw3 and HLA-DRw7. Each PLT was then restimulated with cells from a 35-member unrelated panel. Restimulation values for each PLT were subjected to bimodal clustering analysis. Blocking experiments were performed with other intrafamily and homozygous typing cell PLT after preincubation with B [bone marrow-derived] cell alloantisera. A high correlation was shown (0.881 .ltoreq. r [correlation coefficient] .ltoreq. 1.00) between the HLA-DRw specificity of the priming haplotype and the HLA-DRw specificity of unrelated panel cells that restimulate in PLT. When stimulating cells were absorbed with the corresponding DRw alloantisera or p29,34 heteroantiserum (against B cell specific antigens), PLT restimulation was significantly blocked. The PLT cells treated with antisera showed no effect. HLA-DRw is probably the principal PLT-stimulating determinant.