Further Evidence for the Existence of the p0 Site in the Active Site of Ribonuclease. The Binding of Thymidine 3′, 5′-Diphosphate to Ribonuclease
- 1 October 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 90 (6) , 1685-1690
- https://doi.org/10.1093/oxfordjournals.jbchem.a133644
Abstract
Binding of thymidine 3′,5′-diphosphate to ribonuclease was studied by spectroscopic, kinetic and crystallographic methods. The results show that the 5′-phosphate group is located close to the p0 site in the active site proposed earlier (Mitsui, Y., Urata, Y., Toni, K., & Irie, M. (1978) Biochim. Biophys. Acta 535, 299–308) for stabilizing the complex.Keywords
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