THE 'FREE' AMINO ACIDS OF SOIL

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Abstract
The amount of water-soluble amino acids in the soils studied was low but qualitatively most of the common amino acids were found except cystine and methionine. Extracting the soil with water in the presence of carbon tetrachloride, or carbon tetrachloride containing a complexing agent, increased this amount 25 to 100 times. It is suggested that the role of the carbon tetrachloride was to release ammo acids from microbial or other cellular material in the soil. The complexing agent apparently had little effect on the amount of amino acids extracted with water. The amino acids extracted in the presence of carbon tetrachloride made up 0.1 to 1.0% of the total soil amino acids recovered after acid hydrolysis.

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