Preliminary Crystallographic Analysis of a Proteolytically Modified Form ofE. coliSingle Stranded DNA Binding Protein
- 1 June 1989
- journal article
- research article
- Published by Taylor & Francis in Journal of Biomolecular Structure and Dynamics
- Vol. 6 (6) , 1071-1076
- https://doi.org/10.1080/07391102.1989.10506537
Abstract
A proteolytically modified form of the Escherichia coli single-stranded DNA-Binding protein (SSB) has been crystallized from 15% saturated sodium citrate. Crystals as large as 1.0mm × 0.3mm × 0.2mm were obtained and these diffract beyond 3Å resolution. X-ray photographic analysis demonstrated a rhombohedral unit cell of space group R3 with an equivalent triple centered hexagonal unit cell having dimensions of a = b = 62.9 A and c = 264.3A. These crystals were judged to be adequate for a three dimensional structure determination.Keywords
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