Non-dissociative Small Fragments of Glycinin-T
- 1 March 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 44 (3) , 575-580
- https://doi.org/10.1080/00021369.1980.10863993
Abstract
The small fragments (P) were detected in glycinin-T (tryptic digestion intermediate of glycinin) by acetic acid-urea gel electrophoresis. Urea or 0.5 m acetic acid was needed for dissociation of them from glycinin-T. In the presence of urea, P fragments were separated from ISTs by use of solubility difference and Sephadex G-50 chromatography. Two bands of P fragments were observed on acetic acid-urea gels whereas a single band was on SDS-urea gels suggesting that the molecular weights of the two are almost the same. The fragments were also separable on a column of DEAE Sepharose CL-6B. They were isolated and the molecular weight of them was 9600.This publication has 8 references indexed in Scilit:
- Limited Tryptic Degradation of Urea Denatured GlycininAgricultural and Biological Chemistry, 1979
- Decrease of the Soybean Glycinin Digestibility in Excess Denaturation; Effect of RefoldingAgricultural and Biological Chemistry, 1979
- Formation of Digestion Intermediate of GlycininAgricultural and Biological Chemistry, 1978
- Degradation Sequence of Glycinin by Tryptic HydrolysisAgricultural and Biological Chemistry, 1978
- Tryptic hydrolysis of glycinin and its subunitsJournal of the Science of Food and Agriculture, 1977
- Subunit Structure of Soybean 11S GlobulinAgricultural and Biological Chemistry, 1976
- Purification and stability studies of the 11 S component of soybean proteinsArchives of Biochemistry and Biophysics, 1962
- Studies on the tryptic digestion of myosinBiochimica et Biophysica Acta, 1959