Purification of beta-toxin from Clostridium perfringens type C
- 1 December 1977
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 18 (3) , 741-745
- https://doi.org/10.1128/iai.18.3.741-745.1977
Abstract
.beta.-Toxin was purified about 340-fold from culture supernatant fluid of C. perfringens type C with a yield of about 24% in terms of biologically active .beta.-toxin. The purification involved (NH4)2SO4 fractionation, gel filtration through Sephadex G-100, isoelectrofocusing in a pH 3-6 gradient and immunoaffinity chromatography. The purified .beta.-toxin gave a single band on polyacrylamide gel electrophoresis.This publication has 9 references indexed in Scilit:
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