Inhibition by somatostatin of the vasopressin‐stimulated adenylate cyclase in a kidney‐derived line of cells grown in defined medium
- 24 April 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 169 (2) , 133-137
- https://doi.org/10.1016/0014-5793(84)80304-x
Abstract
LLC‐PK1L cells, a kidney‐derived cell line grown in defined medium, possess a vasopressin‐sensitive adenylate cyclase. Somatostatin was able to inhibit the vasopressin‐induced increase in adenylate cyclase activity, without affecting the basal enzyme activity. This inhibition was competitive. No effect of somatostatin could be detected on [3H]vasopressin binding suggesting an interaction of somatostatin with the vasopressin‐sensitive system distal to the hormone—receptor interaction. At variance with N6‐L‐2‐phenylisopropyladenosine (PIA), GTP did not potentiate the inhibition by somatostatin. The inhibition of the vasopressin stimulation by somatostatin and that by PIA were additive. Changing the composition of the cell growth medium increased the number of vasopressin receptors per cell. Cells with a high number of vasopressin receptors were less sensitive to inhibition by somatostatin. Such results suggested that somatostatin and vasopressin receptors and/or the inhibitory (Ni) and stimulatory (Ns) regulatory transducing components are regulated by different mechanisms.Keywords
This publication has 20 references indexed in Scilit:
- Somatostatin inhibits multireceptor stimulation of cyclic AMP formation and corticotropin secretion in mouse pituitary tumor cells.Proceedings of the National Academy of Sciences, 1982
- Insulin and serum increase the number of receptors for vasopressin in a kidney-derived line of cells grown in a defined medium.Proceedings of the National Academy of Sciences, 1980
- Vasopressin-sensitive adenylate cyclase reversibility of hormonal activationBiochimica et Biophysica Acta (BBA) - General Subjects, 1979
- Immunoreactive and biologically active somatostatin-like material in rat retina.Proceedings of the National Academy of Sciences, 1979
- Isolation and characterization of somatostatin from pigeon pancreas.Proceedings of the National Academy of Sciences, 1979
- Effect of somatostatin on insulin secretion and cAMP content of isolated pancreatic rat isletsMetabolism, 1978
- A cell strain cultured from porcine kidney increases cyclic AMP content upon exposure to calcitonin or vasopressinBiochemical and Biophysical Research Communications, 1978
- Effects of somatostatin on pancreatic exocrine function. Interaction with secretinBiochemical and Biophysical Research Communications, 1975
- A highly sensitive adenylate cyclase assayAnalytical Biochemistry, 1974
- Inhibition of adenosine 3′,5′-monophosphate accumulation in anterior pituitary gland in vitro by growth hormone-release inhibiting hormoneBiochemical and Biophysical Research Communications, 1974