Allele resolution of HLA‐A using oligonucleotide probes in a two‐stage typing strategy

Publisher Website
Google Scholar
Abstract
High‐resolution polymerase chain reaction using sequence‐specific oligonucleotide probes (PCR‐SSOP) typing methods for HLA‐A identification have been established. The four systems, which operate independently of each other, are intended for use as secondary typing systems following HLA‐A identification with a medium‐resolution PCR‐SSOP technique. The systems, all using digoxigenin‐labelled probes, are based on group specific amplifications for resolution of: i) HLA‐A*29 & ‐A*33; ii) HLA‐A*24 & ‐A*30; and iii) HLA‐A*26, ‐A*25, ‐A*11, ‐A*34, ‐A*66 and ‐A*68 alleles, respectively. The fourth system, for the detection of HLA‐A*02 alleles, is a modification of a previously reported PCR‐SSOP subtyping system. The methods have been applied to individuals from the local bone marrow registry and HLA‐A allele frequencies for the Northern Ireland population have been established.