Detection of HLA‐A*24 null alleles by DNA typing methods

Abstract
A number of cases have been identified (seven unrelated individuals from the Northern Ireland bone marrow donor registry and two family groups) where an HLA‐A*24 allele fails to express the normal HLA‐A24 antigen. Family information has revealed common haplotypes with respect to each non‐expressed allele indicating that the occurrence of these mutations has been a recent event. Two methods for the clinical typing of these alleles have been evaluated ‐ PCR‐SSOP and PCR‐SSCP analysis.