Reduction of Rat Thymocyte Interphase Death by Hyperthermia

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Abstract
The effects of hyperthermia on rat thymocytes subjected to 1 kR irradiation were examined. Radiation-induced interphase death (judged by dye staining and ATP decline) observed after 4 h of incubation at 37.degree. C following irradiation was reduced by exposing the cells for 30 min to 1 h to 43.degree. C immediately before or after irradiation. Hyperthermia treatment at a lower temperature (such as 41.degree. C) was effective in inducing cell recovery from radiation damage, although a longer treatment was needed. Hyperthermia administered 2 h after irradiation was ineffective and even enhanced cell death. To elucidate the mechanism underlying the effect, [10]adenine and [32P]orthophosphate were used to estimate changes in ATP metabolism after heat exposure. Heat alone stimulated ATP synthesis and degradation regardless of irradiation. Radiation-induced ATP degradation was markedly depressed by heat treatment. [Heat effects are important in understanding therapeutic value of combined heat and X-ray for cancer.].

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