Effects of prostaglandins E-2 and F-2 on the metabolism of [U-14C]glucose by mouse morulae-early blastocysts in vitro

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Abstract
During 5-h culture in the presence of radioactive glucose, PGE-2 (10 .mu.g/ml) significantly inhibited incorporation of glucose into the acid-soluble glycogen pool. PGE-2 at 1 and 10 .mu.g/ml and PGF-2.alpha. at 1 .mu.g but not 10 .mu.g/ml stimulated incorporation of glucose into non-glycogen macromolecules during culture. However, the utilization of acid-soluble glycogen and other biochemical pools was not affected by the presence of PGs in the medium during 24-h chase culture of pulse-labelled embryos. Carbon dioxide production was significantly suppressed in the presence of PGs but accumulation of lactate was not affected. The results indicate that PGE-2 and PGF-2.alpha., in physiological concentrations, directly influence the metabolism of glucose by preimplantation embryos.