Effect of estradiol, prostaglandin E2 and prostaglandin F2.ALPHA. on incorporation of (3H)uridine by preimplantation mouse embryos in vitro.

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Abstract
To determine whether estrogen and prostaglandins have a direct effect on the preimplantation embryo, the effect of these compounds added to mouse embryos in vitro was examined. Embryos were incubated in culture media containing [3H]uridine with or without the substances to be tested. The addition of estradiol (10-8 M) and prostaglandin F2.alpha. (1 .mu.g/ml or 10 .mu.g/ml) stimulated the incorporation of uridine into RNA. Prostaglandin E2 at a concentration of 10 .mu.g/ml had an inhibitory effect. Estradiol and prostaglandin F2.alpha. may have, in addition to an indirect effect mediated by uterine factors, a direct effect on embryonic growth that activates embryos to implant.