Similar degree of methyl methanesulphonate-induced DNA damage in two clones ofTradescantia differing in mutagenic sensitivity to alkylating agents
- 1 July 1983
- journal article
- research article
- Published by Institute of Experimental Botany in Biologia plantarum
- Vol. 25 (4) , 299-304
- https://doi.org/10.1007/bf02902878
Abstract
In two clones ofTradescantia (4430 and 02) differing in the sensitivity to the mutagenic action of alkylating agents, equimolar doses of [14C] methyl methanesulphonate (MMS) elicited a similar degree of protein, RNA and DNA alkylation and a similar amount of DNA-7-methylguanine and DNA-3-methyladenine in cells of inflorescence. Moreover, in the same clones and tissues the same doses of nonlabelled MMS produced a similar amount of DNA single strand breaks and/or alkali labile sites as measured in alkaline sucrose gradients. None of the DNA lesions followed is therefore decisive for explanation of the different mutagenic sensitivity ofTradescantia clones.This publication has 15 references indexed in Scilit:
- Somatic mutations induced by maleic hydrazide and its potassium and diethanolamine salts in the Tradescantia mutation assayMutation Research Letters, 1982
- Induction of somatic mutations by the promutagen dimethylnitrosoamine in hairs of tradescantia stamenMutation Research/Genetic Toxicology, 1980
- Genetic effects of dimethyl sulfate, diethyl sulfate, and related compoundsMutation Research/Reviews in Genetic Toxicology, 1980
- Rate-Zonal Centrifugation: Quantitative AspectsPublished by Elsevier ,1978
- Intermediates in excision repair by human cells: use of S1 nuclease and benzoylated naphthoylated cellulose to reveal single-strand breaksBiochemistry, 1977
- Comparative effects of ionizing radiation and two gaseous chemical mutagens on somatic mutation induction in one mutable and two non-mutable clones of tradescantiaMutation Research/Environmental Mutagenesis and Related Subjects, 1976
- Comparison of somatic mutation rates induced in tradescantia by chemical and physical mutagensMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1974
- Repair of single-strand breaks and fate of N-7-methylguanine in DNA during the recovery from genetical damage induced by N-methyl-N-nitrosourea in barley seedsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1973
- Tradescantia Stamen Hairs: A Radiobiological Test System Applicable to Chemical MutagenesisPublished by Springer Nature ,1973
- Alkylation of Phosphates and Stability of Phosphate Triesters in DNAEuropean Journal of Biochemistry, 1972