Isolation and amino acid sequence analysis of bovine adrenal 3.beta.-hydroxysteroid dehydrogenase/steroid isomerase
- 1 August 1991
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 30 (33) , 8108-8116
- https://doi.org/10.1021/bi00247a003
Abstract
3 beta-Hydroxysteroid dehydrogenase/steroid isomerase has been purified to homogeneity from bovine adrenal glands. A single protein of molecular weight 42,090 +/- 40 containing both enzyme activities has been isolated. Approximately 86% of the amino acid sequence of the bovine adrenal 3 beta-hydroxysteroid dehydrogenase/steroid isomerase has been obtained by sequencing peptides isolated from digests with trypsin and lysyl endopeptidase and by chemical cleavage with CNBr. The sequence obtained is identical with that of the deduced amino acid sequence of the bovine ovarian 3 beta-hydroxysteroid dehydrogenase/steroid isomerase [Zhao et al. (1989) FEBS Lett. 259, 153-157], with the exception that the N-terminal methionine residue found in the bovine ovarian sequence is not present in the mature bovine adrenal enzyme. On the basis of the primary structure and comparisons with other NAD+ binding proteins, we propose a structural model of the bovine adrenal 3 beta-hydroxysteroid dehydrogenase/steroid isomerase localizing the NAD+ binding site as well as the membrane-anchoring segment.Keywords
This publication has 38 references indexed in Scilit:
- Affinity alkylation of human placental 3β -hydroxy-5-ENE-steroid dehydrogenase and steroid 5→4-ene-isomerase by 2α-bromoacetoxyprogesterone: Evidence for separate dehydrogenase and isomerase sites on one proteinThe Journal of Steroid Biochemistry and Molecular Biology, 1990
- Molecular cloning, cDNA structure and predicted amino acid sequence of bovine 3β‐hydroxy‐5‐ene steroid dehydrogenase/Δ5‐Δ4 isomeraseFEBS Letters, 1989
- Human placental 3β-hydroxy-5-ene-steroid dehydrogenase and steroid 5 → 4-ene-isomerase: Purification from microsomes, substrate kinetics, and inhibition by product steroidsJournal of Steroid Biochemistry, 1988
- Testicular and adrenal 3β-hydroxy-5-ene-steroid dehydrogenase and 5-ene-4-ene isomeraseJournal of Steroid Biochemistry, 1987
- Prediction of the occurrence of the ADP-binding βαβ-fold in proteins, using an amino acid sequence fingerprintJournal of Molecular Biology, 1986
- Structures of two cockroach neuropeptides assigned by fast atom bombardment mass spectrometryBiochemical and Biophysical Research Communications, 1984
- On the influence of hydrophobicity in the sims spectra of amino acids in glycerol matrixBiochemical and Biophysical Research Communications, 1984
- Extended superfamily of short alcohol‐polyol‐sugar dehydrogenases: structural similarities between glucose and ribitol dehydrogenasesFEBS Letters, 1984
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Enzymic isomerization of Δ5-3-ketosteroidsBiochimica et Biophysica Acta, 1954