THE NATURE OF TWO COMPONENTS OF PIG MUCOSAL HEPARIN, SEPARATED BY ELECTROPHORESIS IN AGAROSE GEL
- 30 June 1967
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 45 (7) , 1015-1020
- https://doi.org/10.1139/o67-117
Abstract
Two components of pig mucosal heparin were separated by agarose-gel electrophoresis and recovered from the gels. The slower moving component had the higher anticoagulant activity and intrinsic viscosity. The faster moving component had the higher sulfate : carboxyl ratio. Each component was degraded by self-hydrolysis to N-desulfated heparin, deaminated with nitrous acid, and hydrolyzed with formic acid. Two-dimensional electrophoresis and chromatography on thin layers of cellulose showed that each component had two uronic acids with the characteristics of glucuronic and iduronic acids.This publication has 11 references indexed in Scilit:
- Micro-electrophoresis of mucopolysaccharides on agarose gelJournal of Chromatography A, 1963
- Incorporation of 14C and 3H from Nucleotide Sugars into a Polysaccharide in the Presence of a Cell-free Preparation from Mouse Mast Cell TumorsJournal of Biological Chemistry, 1963
- Identification of uronic acids in mucopolysaccharidesArchives of Biochemistry and Biophysics, 1963
- Paper Chromatography of Several Classes of Compounds: Correlated Rf Values in a Variety of Solvent SystemsAnalytical Chemistry, 1963
- Chemical and Pharmacological Studies on N-Resulfated HeparinExperimental Biology and Medicine, 1962
- The uronic acid of heparinBiochemical and Biophysical Research Communications, 1962
- A Method for the Separation of Acid Mucopolysaccharides: Its Application to the Isolation of Heparin from the Skin of RatsJournal of Biological Chemistry, 1961
- Studies on fractionated heparinArchives of Biochemistry and Biophysics, 1961
- On the relationship between the sulphur content and the anticoagulant activity of heparin preparationsBiochemical Journal, 1939