Studies on cardiolipin biosynthesis in Mycobacterium smegmatis

Abstract
Supplementation of a growth medium with 5% glucose stimulated the formation of cardiolipin and phosphatidylethanolamine 5- and 3-fold, respectively, in M. smegmatis. The presence of both cytidine diphosphate diglyceride and phosphatidylglycerol pathways of biosynthesis of cardiolipin in cell-free extracts was demonstrated. The enzymes were localized in the fractions which contained membranes. Isonicotinic acid hydrazide and streptomycin sulfate inhibited the formation of cardiolipin.

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