A hydrolytic procedure for the identification and estimation of individual phospholipids in biological samples

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Abstract
The products of selective hydrolytic degradation are separated by paper chromatography and their phosphorus contents measured. From these, the nature and amounts of the phospholipids originally present are inferred, appropriate corrections being based upon the hydrolysis of reference compounds. By this method, the distribution of lecithin, phosphatidylethanolamine, phosphatidylserine, monophosphoinositide, polyglycerol phospholipid,(cardiolipin), "phosphatidic acid," choline plasmalogen, ethanolamine plasmalogen, serine plasmalogen and sphingomyelin has been measured in 6 tissues of the sheep.