Differential Dichrome Staining of Tissue Culture Monolayers: Alternate Dyes and Possible Mechanism
- 1 January 1978
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 53 (6) , 315-320
- https://doi.org/10.3109/10520297809111953
Abstract
A polyacid-dependent dichrome was devised which differentiates epithelial [bovine dental organ] from mesenchymal [bovine dental pulp] cells in young dividing primary cultures. Epithelial cells and colonies and nuclei are stained with metanil yellow, the stain is fixed and differentiated with phosphotungstic acid and the mesenchymal elements are stained with toluidine blue. Several other dyes are tested for substitution in this method. Biebrich scarlet and aniline blue were substituted for metanil yellow; Bismarck brown T, Janus green B, crystal violet, and neutral red were substituted for the basic dye.Keywords
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