Thermal Enhancement of X-Ray-Induced DNA Crosslinking

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Abstract
Ionizing radiation appears to crosslink nuclear DNA with chromosomal proteins. Important cellular processes such as transcription and DNA replication are likely to be compromised as a result of the DNA crosslinking. Heat treatment (43.degree. C) of mouse leukemia cells (L1210) before X-irradiation (50 Gy [gray]) caused a doubling of the radiation-induced DNA crosslinking as measured by alkaline elution technique. By using proteinase K, a very active protease, to eliminate DNA-protein crosslinking in the alkaline elution assay, the thermally enhanced DNA crosslinking was attributed to an increase in DNA-protein crosslinking. However, utilizing a protein radiolabel technique under conditions of increased DNA-protein crosslinking, the amount of protein left on the filter in the elution assay was not increased. Qualitative rather than large quantitative differences in the crosslinked chromosomal proteins probably exist between irradiated cells and cells treated with heat prior to irradiation.

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