Restriction endonuclease mapping of linear unintegrated proviral DNA of bovine leukemia virus
- 1 April 1981
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 38 (1) , 27-33
- https://doi.org/10.1128/jvi.38.1.27-33.1981
Abstract
A detailed restriction map was deduced for the genome of the exogenous bovine leukemia virus. The cleavage sites for nine restriction enzymes were mapped. The unintegrated linear viral DNA intermediate that is produced by infection of permissive cells with bovine leukemia virus was isolated. The linear viral DNA had a unique restriction map, indicating that it is not a set of random circular permutations of the RNA genome. From hybridization with a 3'-enriched probe, the DNA restriction map was aligned relative to the 5'-to-3' orientation of the viral RNA. Restriction enzyme analysis of integrated bovine leukemia virus information present in animals with enzootic bovine leukosis provided evidence for the existence of genetic variants of the virus.This publication has 17 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Genomic integration of bovine leukemia provirus: comparison of persistent lymphocytosis with lymph node tumor form of enzootic.Proceedings of the National Academy of Sciences, 1980
- Integration of bovine leukemia virus DNA in the bovine genome.Proceedings of the National Academy of Sciences, 1979
- Restriction endonuclease cleavage of linear and closed circular murine leukemia viral DNAS: Discovery of a smaller circular formCell, 1979
- Isolation and organization of calf ribosomal DNANucleic Acids Research, 1979
- Mapping unintegrated avian sarcoma virus DNA: Termini of linear DNA bear 300 nucleotides present once or twice in two species of circular DNACell, 1978
- Mapping of linear and circular forms of mouse mammary tumor virus DNA with restriction endonucleases: evidence for a large specific deletion occurring at high frequency during circularization.Proceedings of the National Academy of Sciences, 1978
- Covalently closed circular DNA of avian sarcoma virus: Purification from nuclei of infected quail tumor cells and measurement by electron microscopy and gel electrophoresisJournal of Molecular Biology, 1976
- Enhancement and inhibition of avian sarcoma viruses by polycations and polyanionsVirology, 1969
- A membrane-filter technique for the detection of complementary DNABiochemical and Biophysical Research Communications, 1966