Carbon-13 NMR spectroscopic study of teichuronic acid from Micrococcus luteus cell walls. Comparison of the polysaccharide isolated from cells with that synthesized in vitro

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Abstract
Teichuronic acid isolated from the cell walls of M. luteus was examined by natural-abundance 13C-NMR spectroscopy. Proton-decoupled and proton-coupled spectra were obtained for native teichuronic acid and after the teichuronic acid was oxidized with periodate and reduced with borohydride. The spectra are consistent with the structure [ManNAcUAp-(.beta.-1,6)-Glcp-(.alpha.-1,4)]n. Teichuronic acid synthesized in vitro from suitable substrates by the particulate enzyme fraction obtained from M. luteus yielded a 13C-NMR spectrum which is indistinguishable from that of the native teichuronic acid, indicating a structural identity of the teichuronic acid synthesized in vitro with that isolated from cell walls.