Effects of Sucrose Concentration on the Photosynthetic Ability of Rose Shoots In Vitro

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Abstract
Reducing the concentration of sucrose in the culture medium over successive subcultures has been tested as a method for increasing the ability of rose shoots grown in vitro (Rosa cvs Iceberg and Peace) to take up CO2. Shoots maintained on ‘constant’ 10, 20 and 40 g I−1 sucrose showed decreased levels of CO2 uptake at higher sucrose concentrations, although cv. Peace grew least at 10 g l−1 and showed correspondingly low amounts of CO2 uptake compared with 20 and 40 g l−1. Both cultivars died when sucrose was omitted from the medium. As sucrose concentration was reduced in the medium, so CO2 uptake of shoots initially cultured on 20 and 40 g l−1 sucrose was found to increase, although a concentration of 10 gl −1 sucrose seemed to be limiting, below which the growth and chlorophyll levels of shoots declined.