Phosphatidylcholine‐phospholipase C mediates the induction of nerve growth factor in cultured glial cells
- 15 May 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 364 (3) , 301-304
- https://doi.org/10.1016/0014-5793(95)00414-5
Abstract
Addition of phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) to cultured glial cells increased the levels of nerve growth factor (NGF) mRNA and the amount of cell-secreted NGF. The effect of PC-PLC was 2.5 times higher than that elicited by 4β-phorbol 12β-myristate 13α-acetate. In cells in which protein kinase C (PKC) was fully inhibited or downregulated, the effect of PC-PLC was reduced - though still evident - and similar to that exerted by sphingosine. Results thus indicate that PC-PLC induces the synthesis of NGF by glial cells by a PKC-dependent and PKC-independent mechanisms.Keywords
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