High-throughput assay for small molecules that modulate zebrafish embryonic heart rate
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- 18 September 2005
- journal article
- Published by Springer Nature in Nature Chemical Biology
- Vol. 1 (5) , 263-264
- https://doi.org/10.1038/nchembio732
Abstract
To increase the facility and throughput of scoring phenotypic traits in embryonic zebrafish, we developed an automated micro-well assay for heart rate using automated fluorescence microscopy of transgenic embryos expressing green fluorescent protein in myocardium. The assay measures heart rates efficiently and accurately over a large linear dynamic range, and it rapidly characterizes dose dependence and kinetics of small molecule-induced changes in heart rate. This is the first high-throughput micro-well assay for organ function in an intact vertebrate.Keywords
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